Translation of preclinical results into effective cell-based therapies remains poor, highlighting the need for accurate human-emulation systems. Recently, 3D in vitro systems, termed organoids or spheres, that contain an important number of stem cells and allow the recreation of similar to the in vivo conditions, have been successfully generated from both dental epithelial and mesenchymal tissues. Organoids might be valuable sources of dental stem cells for regenerative purposes. Similarly, they represent valuable tools for studying the effects of novel pharmaceutical products and materials to dental tissues before their clinical application. Miniaturized “organ-on-chip” devices are based on recent technological advancements in microfluidics. These devices successfully emulate human pathophysiological conditions of specific tissues and organs in vitro. Microfluidics have been used for the first time to analyze the role of innervation and vascularization in dental tissues and stem cells. These devices might be also important to understand the interconnection of teeth with other organs and study their responses to the various dental pathologies upon bacterial attack. Graphene-based sensors, temporary printed at the surface of enamel, permitted the detection of oral bacteria and the evaluation of food properties such as pH, temperature and sugar levels. Such sensors represent excellent tools for the refined control and understanding of oral environment that will greatly help preventive dentistry. The combination of these biotechnological platforms gave rise to pioneering techniques that are revolutionizing medicine and greatly promise for improved dental care in the near future. In this lecture, a good amount of novel findings that is the result of fruitful collaborations between the Institute of Oral Biology with the Technological Institute of Milano and ETHZ, will be presented.