Prof. Dr. Carsten Sönnichsen

Physical Chemistry, University of Mainz

Plasmonic Nanoparticles as sensor for single molecules

Abstract

We have developed an alternative to dye labeling for single molecule experiments: we utilize plasmonic gold nanoparticles to detect single unlabeled proteins with high temporal resolution (ms to µs). This allows for monitoring the dynamic evolution of DNA hybridization or protein binding. The technique resolves equilibrium coverage fluctuations, opening a window into Brownian dynamics of unlabeled macromolecules. Therefore, our method enables the study of DNA or protein folding dynamics, protein adsorption processes, and kinetics as well as non-equilibrium soft matter dynamics on the single molecule level without need for labeling.

 



Date: 4/9/2014
Time:12:00 (coffee & cookies will be served at 11:45)
Place:FORTH Seminar Room 1