Prof. Alexandre A. Mironov, MD, PhD, DSc

Group of Intracellular Transport and Tumorigenesis. Head

Modern 3D electron microscopy: State of art and biological applications



The final goal of any morphological analysis of a biological sample, whether it is an organism, organ, tissue, cell, organelle, or molecule is to get an averaged three-dimensional model of the structure studied and to determine the chemical composition of the listed items. For a long time, this problem was solved on the basis of the two-dimensional design. However, in recent years, several breakthroughs in the field of electron microscopy (EM) allowed to develop several new methods of EM for the examination of three-dimensional organization of organelles, cells and tissues. On the other hand, in many cases structures and events visible inside cells are very transient and rare. Therefore, biological studies have relied on two complementary microscope technologies – light (fluorescence) microscopy and electron microscopy. Light microscopy is used to study phenomena at a global scale to look for unique or rare events, and it also provides an opportunity for live imaging, whereas the forte of electron microscopy is the high resolution. Traditionally light and electron microscopy observations are carried out in different populations of cells/tissues and a ‘correlative’ inference is drawn. The advent of true correlative light-electron microscopy has allowed high-resolution imaging by electron microscopy of the same structure observed by light microscopy, and in advanced cases by video microscopy. Thus a rare event captured by low-resolution imaging of a population or transient events captured by live imaging can now also be studied at high resolution by electron microscopy. Correlative light electron microscopy (CLEM; Polishchuk et al., 2000; Mironov and Beznoussenko, 2009).) is one of three the most promisible directions of the modern electron microscooy, which are developing now, namely: 1) three-dimensional electron microscopy (3DEM); 2) CLEM; 3) electron cryomicroscopy. Now, CLEM and is one of the most potent tools for the EM analysis of rare events. Here, I will present recent achievments in the fiedl of 3DEM and CLEM.

Date: 21/7/2014
Time:12:00 (coffee & cookies will be served at 11:45)
Place:FORTH Seminar Room 1