In the first part of this talk a brief review on the non linear laser imaging techniques will be displayed. In particular, two photon fluorescence microscopy, lifetime imaging, multispectral imaging, second harmonic generation microscopy principles will be described.

In the second part of the talk there will be an overview on the applications of these techniques in the field of biomedical imaging.  In particular, tumor detection in tissue imaging applications will be shown in different fields, from urology to gastrointestinal surgery, dermatology and brain surgery.

Morpho-functional characterization of tissue pathologies will be displayed as an interesting tool for tumor early diagnosis.  Other type of non tumor disease applications will be also shown together with the demonstration on using laser imaging for follow up of laser therapies.

In the last part of the talk, a fiber based endoscope based on multidimensional spectral detection (one photon fluorescence, lifetime and Raman detection) will be described with particular applications of tumor detection.