Direct Laser Fabrication of Biomimetic Scaffolds

Contact Person(s):
Dr. Emmanuel Stratakis Dr. Anthi Ranella

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The aim is to investigate cell response of different kinds of cells on laser engineered biomimetic 3D scaffolds of different micro/nano topographies and/or surface energies.


Research Topics
Tissue Engineering
Ultrafast Laser Micro- and Nano- processing



The main aim of this research activity is to investigate the adhesion and bioactivity of different cell types on biomimetically modified 3-dimensional substrates, such as Si and different types of polymers, in order to develop scaffolds for potential use in tissue engineering applications. To this end, we use ultrafast laser structured Si wafers, comprising dual scale roughness in the micro- and nano- length scales. In this respect, the behaviour of fibroblast and neurons cultured on substrates prepared to exhibit gradient morphologies and/or chemistries was investigated. We have shown that it is possible to preferentially tune cell adhesion and growth, through choosing proper combinations of topography and chemistry of 3D micro/nano structures.




A. Gravanis, I. Charalampopoulos, University Of Crete, Medical School
I. Athanassakis, University Of Crete, Dept. of Biology
A. Samara, E. Anastasiadou, S. Pagkakis, Biomedical Research Foundation of the Academy of Athens
A. G. Kanaras, Physics & Astronomy, University of Southampton.
C. Hosokawa, Biointerface Research Group, Health Research Institute (HRI) National Institute of Advanced Industrial Science and Technology (AIST)



  • Biomimetic micro/nanostructured functional surfaces for microfluidic and tissue engineering applications
    E. Stratakis, A. Ranella, and C. Fotakis, Biomicrofluidics, 5, 013411, 2011

  • Direct laser writing of 3D scaffolds for neural tissue engineering applications
    V. Melissinaki, A. A. Gill, I. Ortega, M. Vamvakaki, A. Ranella, J. W. Haycock, C. Fotakis, M. Farsari, and F. Claeyssens, Biofabrication, Vol. 3, 045005, 2011

  • Controlling cell adhesion via replication of laser micro/nano-textured surfaces on polymers
    N. Koufaki, A. Ranella, K. E Aifantis, M. Barberoglou, S. Psycharakis, C. Fotakis, E. Stratakis , Biofabrication, 3, 045004, 2011

  • Silicon Scaffolds Promoting 3D Neuronal Web of Cytoplasmic Processes
    E.L. Papadopoulou, A. Samara, M. Barberoglou, A. Manousaki, S.N. Pagakis, E. Anastasiadou, C. Fotakis and E. Stratakis, Tissue Engineering Part C, 16, 497, 2010

  • Tuning cell adhesion by controlling the roughness and wettability of 3D micro/nano silicon structures
    A. Ranella, M. Barberoglou, S. Bakogianni, C. Fotakis, E. Stratakis, Acta Biomaterialia, 6, 2711, 2010

  • Laser-based micro/nanoengineering for biological applications
    E. Stratakis, A. Ranella, M. Farsari, and C. Fotakis, Progress in Quantum Electronics, 33, 127, 2009

  • Applications of ultrafast lasers in materials processing: Fabrication on self-cleaning surfaces and scaffolds for tissue engineering
    C. Fotakis, M. Barberoglou, V. Zorba, E. Stratakis, E. L. Papadopoulou, A. Ranella, K. Terzaki, and M. Farsari, Proceedings of SPIE, Laser Physics and Applications 2008, 2008

  • Novel Aspects of Materials Processing by Ultrafast Lasers: From Electronic to Biological and Cultural Heritage Applications
    C. Fotakis, V. Zorba, E. Stratakis, P. Tzanetakis, I. Zergioti, D. G. Papagoglou, K. Sambani, G. Filippidis, M. Farsari, P. Pouli, G. Bounos, S. Georgiou, Journal of Physics: Conference Series, 59, 266, 2007

Project Members
Dr. Emmanuel Stratakis
Ms. Chara Simitzi
Ms. Xristina Yiannakoy
Mr. Miron Krassas
Dr. Anthi Ranella
Prof. Costas Fotakis

Last Updated:  7/5/2013