Non linear imaging microscopy for biological applications


Contact Person(s):
Dr. George Filippidis


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Non-linear (Multi-Photon Excitation Fluorescence (MPEF), Second-Harmonic Generation (SHG) and Third Harmonic Generation (THG)) microscopy measurements are implemented for the in vivo detailed imaging of structures and processes of various biological specimens

Research Topics
Non-linear Microscopy

Abstract

A prototype workstation that combines nonlinear imaging modalities (MPEF, SHG, THG) and nanosurgery capabilities in a single instrument has been developed. Femtosecond laser pulses are utilized for excitation. This home-made nonlinear microscope has been successfully employed for the in vivo sub-cellular investigation of complex biological processes (embryogenesis, neuronal degeneration, ageing) and the extraction of valuable structural information from various samples (cancer cells, mouse embryos, C. elegans).



Collaborators

Nektarios Tavernarakis IMBB-FORTH

http://www.tavernarakislab.gr/

Irene Athanassakis UoC-Biology Department

http://www.biology.uoc.gr/el/personnel/38


Publications

  • Compact ultrafast semiconductor disk laser: targeting GFP based nonlinear applications in living organisms
    Rodrigo Aviles-Espinosa, George Filippidis, Craig Hamilton, Graeme Malcolm, Kurt J. Weingarten, Thomas Südmeyer, Yohan Barbarin, Ursula Keller, Susana I.C.O Santos, David Artigas, and Pablo Loza-Alvarez, Biomedical Optics Express, 2, 739, 2011

  • Cell tracking in live Caenorhabditis elegans embryos via Third Harmonic Generation imaging microscopy measurements
    George J. Tserevelakis, George Filippidis, Evgenia V. Megalou, Costas Fotakis, and Nektarios Tavernarakis, Journal of Biomedical Optics, 16(4), 000000 , 2011

  • Imaging Caenorhabditis elegans embryogenesis by third-harmonic generation microscopy
    G.J. Tserevelakis, G. Filippidis, A.J. Krmpot, M. Vlachos, C. Fotakis,c, N. Tavernarakis, Micron, 41, 444, 2010

  • In vivo imaging of cell morphology and cellular processes in Caenorhabditis elegans, using non-linear phenomena
    G. Filippidis, E.J. Gualda, M. Mari, K. Troulinaki, C. Fotakis, N.Tavernarakis, Micron, 40, 876, 2009

  • In vivo polarization dependant Second and Third harmonic generation imaging of Caenorhabditis elegans pharyngeal muscles
    G. Filippidis, K. Troulinakis, C. Fotakis, N. Tavernarakis, Laser Physics, 19, 1475, 2009

  • In vivo imaging of neurodegeneration in Caenorhabditis elegans by third harmonic generation microscopy
    E.J. Gualda, G. Filippidis, M. Mari, G. Voglis, M. Vlachos, C. Fotakis, N. Tavernarakis, Journal of Microscopy, 232, 270, 2009

  • In vivo imaging of cellular structures in Caenorhabditis elegans by combined TPEF, SHG and THG microscopy
    E.J. Gualda, G. Filippidis, G. Voglis, M. Mari, C. Fotakis, N. Tavernarakis, Journal of Microscopy-Oxford, 229, 141, 2008

  • Photophysical Properties of Hypericum Perforatum L. Extracts - Novel Photosensitizers for PDT
    D. Skalkos, E. Gioti, C.G. Stalikas, H. Meyer, T.G. Papazoglou, G. Filippidis, Journal of Photochemistry and Photobiology B, 82, 146, 2006

  • Imaging of Caenorhabditis elegans samples and sub-cellular localization of new generation photosensitizers for photodynamic therapy, using non-linear microscopy
    G. Filippidis, C. Kouloumentas, D. Kapsokalyvas, G. Voglis, N. Tavernarakis, and T. G. Papazoglou, Journal of Physics D, 38, 2625, 2005

  • Imaging of Caenorhabditis elegans neurons by Second Harmonic Generation and Two-Photon Excitation Fluorescence
    G. Filippidis, C. Kouloumentas, G. Voglis, F. Zacharopoulou, T. G. Papazoglou, N. Tavernarakis, Journal of Biomedical Optics, 10, 024015, 2005

  • Does Hypericum perforatum L. extract show any specificity as photosensitizer for HL-60 leukemic cells and cord blood hemopoietic progenitors during photodynamic therapy?
    D. Kapsokalyvas, H. Dimitriou, D. Skalkos, G. Konstantoudakis, G. Filippidis, E. Stiakaki, T. Papazoglou, and M. Kalmanti, Journal of Photochemistry and Photobiology B, 80, 208, 2005

Project Members
Prof. Costas Fotakis
Dr. Mari Meropi
Dr. George Filippidis



Last Updated:  10/11/2017
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