Non-linear Microscopy

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Our main research interest concerns the in vivo elucidation of molecular mechanisms and biological processes by employing various spectroscopic techniques with emphasis on advanced microscopic modalities. The investigation of in vivo cellular and sub-cellular activities, by means of non destructive spectroscopic techniques, can provide valuable information related to fundamental biological problems, leading to the development of innovative methodologies for the early diagnosis and treatment of several diseases. Non-linear (Multi Photon Excitation Fluorescence (MPEF), Second Harmonic Generation (SHG), Third Harmonic Generation (THG)) imaging modalities were implemented for elucidating structural and anatomical changes of various biological samples and for probing functions and developmental processes in vivo at the microscopic level. The in vivo sub-cellular investigation of complex biological activities (embryogenesis, neuronal degeneration, aging, cell activation and differentiation) and the extraction of valuable structural and morphological information from various samples (cancer cell lines, mouse embryos, C. elegans, BV-2 microglia cells, T cells, cancer tissue) have been achieved.


Additionally, these non-linear image contrast modalities have been successfully employed as novel diagnostic tools for Cultural Heritage (CH) studies. These high resolution minimally invasive techniques have the potential to provide new insights and information for the exact thickness determination and composition identification as a function of depth of different multi layer CH objects.


Contact Person(s):
Dr. George Filippidis
Prof. Costas Fotakis

Last Updated:  13/11/2017