Dr. Spyros Petrakis

Institute of Applied Biosciences (INAB) Centre For Research And Technology Hellas (CERTH)

Genetically modified human stem cells; applications in biotechnology and disease modeling applications in biotechnology and disease modeling

Abstract

 

Genetically modified human stem cells;

applications in biotechnology and disease modeling

 

Dr. Spyros Petrakis

Institute of Applied Biosciences (INAB)

Centre For Research And Technology Hellas (CERTH)

57001 Thessaloniki, GREECE

Tel: +30 2311 257527

email: spetrak@certh.gr

 

Abstract

Human stem cells are excellent tools for biotechnological applications; they can be easily expanded, genetically modified and used for regenerative purposes or disease modeling. Here, we present  a series of stem cell lines modified with the Sleeping Beauty transposon technology, a non-viral molecular tool with minimum cytotoxicity and high efficiency. Mesenchymal stem cells (MSCs) modified with PSMB5 or hTERT genes may indicate mechanisms of cellular senescence. MSCs carrying biosensor genes can be incorporated in medical devices and used for continuous glucose monitoring in diabetes patients. Additionally, MSCs or small-molecule neural precursor cells (smNPCs) overexpressing mutant polyQ proteins in an inducible manner may model protein aggregation in neurodegenerative diseases. Cultured in scaffolds, these cells may form 3D neuronal structures with similar alterations to a pathological brain tissue.



Date: 21/6/2017
Time:12:00 (coffee & cookies will be served at 00:00)
Place:FORTH Seminar Room 2